Phytochemical analysis, Protein and DNA estimation and Antimicrobial Activity of Azadirachta indica (Neem) | Original Article

ABSTRACT:

ABSTRACT

The present study was aimed to investigate the phytochemical screening and antimicrobial assessment of Azadirachta indica leaf extract. These plants were collected; aqueous and alcoholic extracts were prepared. The phytochemical analysis of extracts were performed, and antimicrobial activity of aqueous and alcoholic extracts of plant leaf material at dose of 100 mg/ml and 200 mg/ml concentrations against two strains were done. These extract were studied through agar diffusion method against Pseudomonas fluorescens an aerobic, gram-negative, ubiquitous organism present in agricultural soils and well adapted to grow in the rhizosphere and Alterneria solani fungal pathogen that produces a disease in tomato and potato plants called early blight. The phytochemical study inferred the presence of secondary metabolites which assist their herbal properties. For a long period of time, plants have been a valuable source of medicines. Phytochemical analysis of the alcoholic extracts revealed the presence of alkaloids, glycosides, flavonoids, steroids, tannins and reducing sugars. The occurrence of these biologically active chemicals in the selected plants may justify their wide usage in traditional medicine.